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Identification of glutathione-overproducing mutants via acrolein resistance A genetically diverse S

The membranes were then incubated for 5 min for chemiluminescence in Clarity Western ECL Substrate (Bio-Rad, 1705060) and visualized by Amersham Imager 600 (GE Healthcare Life Sciences)

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It is likely that these differences in the two methods arise from differences in sensitivity, given that our first mass spec experiment failed to identify any proteins when conducted using the same amount of input material as used for the redox arrays